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[211029] 저널 발표 Human microglia states are conserved across experimental models and regulate neural stem cell responses in chimeric organoids

정선우 │ 2021-10-28

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Cell Stem Cell
Available online 17 September 2021
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Human microglia states are conserved across experimental models and regulate neural stem cell responses in chimeric organoids

Summary

Microglia are resident macrophages in the brain that emerge in early development and respond to the local environment by altering their molecular and phenotypic states. Fundamental questions about microglia diversity and function during development remain unanswered because we lack experimental strategies to interrogate their interactions with other cell types and responses to perturbations ex vivo. We compared human microglia states across culture models, including cultured primary and pluripotent stem cell-derived microglia. We developed a “report card” of gene expression signatures across these distinct models to facilitate characterization of their responses across experimental models, perturbations, and disease conditions. Xenotransplantation of human microglia into cerebral organoids allowed us to characterize key transcriptional programs of developing microglia in vitro and reveal that microglia induce transcriptional changes in neural stem cells and decrease interferon signaling response genes. Microglia additionally accelerate the emergence of synchronized oscillatory network activity in brain organoids by modulating synaptic density


Highlights

Microglia culture models differentially attenuate and preserve gene signatures

Brain organoid microenvironment preserves microglial homeostatic state

Genes in the interferon response pathway are attenuated in the presence of microglia

Microglia decrease double-stranded DNA breaks and cell stress in radial glia

https://doi.org/10.1016/j.stem.2021.08.015




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