Supplemental Movies 1. 3D-SIM images of littermate microvessels in sliced brain.
Coronal serial sections of brains from 8-month-old littermate mice were stained with anti-GLUT1 (red).
Brain slices of littermate make 3D volume image with alpha blending function.
Littermate showed a hole enclosed thick and strong vessel. Scale bars = 2 μm. 3D depth, 4.65 μm in littermate
Supplemental Movies 2. 3D-SIM images of 5XFAD microvessels in sliced brain.
Coronal serial sections of brains from 8-month-old 5XFAD mice were co-stained with anti-GLUT1 (red) and anti-Aβ (green; 4G8) antibodies. Brain slices of 5XFAD make 3D volume image with alpha blending function. 3D-SIM image showed apparently the cerebral capillary of 5XFAD mice was more damaged and had scratch all over the vessel.
Scale bars = 2 μm. 3D depth, 5.45 μm in 5XFAD